DNA Fragmentation and Vitamin K2 Levels in Sperm Maturation and Apoptosis
Fragmentasi DNA dan Tingkat Vitamin K2 dalam Pematangan Sperma dan Apoptosis
DOI:
https://doi.org/10.21070/ijhsm.v2i2.117Keywords:
Vit K, Apoptosis, DNA Fragmentation, Biomarker, ROSAbstract
Background: There is mounting evidence that vitamin K2 (menaquinones) plays a role as a hormone and transcriptional regulator, which has raised interest in its possible impact on male reproductive health. The vitamin K series as a whole has a long history of therapeutic use around the world. Because of its essential function in removing damaged germ cells and preserving the quality of spermatozoa, vitamin K may have an antioxidant impact that is advantageous to spermatogenesis, a process regulated by apoptosis. Aim of the study: Association of DNA fragmentation as an apoptotic marker and Vit K2 levels with sperm maturation. Patients, materials and methods: Sixty-seven infertile men were included in this Cross-sectional study. All the patients underwent Vit K assessment by ELISA, Seminal fluid analysis according to WHO 2021 and DNA fragmentation test using aniline blue staining. Results: The study found highly significant inverse correlation between vit K2 level and DNA fragment proportion of the study sample of semen but there is inverse insignificant correlation of fragmentation with sperm motility while other semen parameters showed insignificant positive correlation including concentration, vitality and normal morphology. Progressive motile sperms (AB) and immotile sperms (D) were found with high significant difference between sperm DNA fragmentation and non-sperm DNA fragmentation. Also, there were no significant differences between infertile men with and without sperm DNA fragmentation in age, smoking, alcohol conception, varicocele and type of infertility. Conclusions: The findings revealed a highly significant negative correlation between vitamin K2 levels and sperm with DNA fragmentation, as well as a highly significant positive correlation between vitamin K2 levels and sperm without DNA fragmentation. The findings indicate that vitamin K2 may play a protective function in protecting sperm DNA integrity.
Highlights :
- Vitamin K2 Protects Sperm DNA – Higher K2 levels correlate with lower DNA fragmentation.
- Minimal Impact on Motility – Fragmentation shows weak links to sperm motility and other parameters.
- No Link to Lifestyle – Factors like smoking and varicocele show no significant effect.
Keywords : Vit K, Apoptosis, DNA Fragmentation, Biomarker, ROS
References
[1] M. E. Thoma, A. C. McLain, J. F. Louis, et al., “Prevalence of Infertility in the United States as Estimated by the Current Duration Approach and a Traditional Constructed Approach,” Fertil. Steril., vol. 99, no. 5, pp. 1324–1331.e1, 2013, doi: 10.1016/j.fertnstert.2012.11.037.
[2] Y. Qiu, H. Yang, C. Li, and C. Xu, “Progress in Research on Sperm DNA Fragmentation,” Med. Sci. Monit., vol. 26, p. e918746–1, 2020, doi: 10.12659/MSM.918746.
[3] R. Asadi, R. Ghahremani, A. Abdolmaleki, and F. Rajaei, “Role of Sperm Apoptosis and Oxidative Stress in Male Infertility: A Narrative Review,” Int. J. Reprod. Biomed., vol. 19, no. 6, p. 493, 2021, doi: 10.18502/ijrm.v19i6.9371.
[4] J. Ribas-Maynou, A. Garcia-Peiro, A. Fernandez-Encinas, et al., “Double Stranded Sperm DNA Breaks, Measured by Comet Assay, Are Associated with Unexplained Recurrent Miscarriage in Couples Without a Female Factor,” PLoS One, vol. 7, no. 9, p. e44679, 2012, doi: 10.1371/journal.pone.0044679.
[5] D. Evenson and R. Wixon, “Meta-Analysis of Sperm DNA Fragmentation Using the Sperm Chromatin Structure Assay,” Reprod. Biomed. Online, vol. 12, no. 4, pp. 466–472, 2006, doi: 10.1016/S1472-6483(10)62000-7.
[6] S. Akbari and A. A. Rasouli-Ghahroudi, “Vitamin K and Bone Metabolism: A Review of the Latest Evidence in Preclinical Studies,” Biomed. Res. Int., vol. 2018, 2018, doi: 10.1155/2018/4629383.
[7] Y. Hirota and Y. Suhara, “New Aspects of Vitamin K Research with Synthetic Ligands: Transcriptional Activity via SXR and Neural Differentiation Activity,” Int. J. Mol. Sci., 2019, doi: 10.3390/ijms20123006.
[8] W. Shum, B. L. Zhang, A. S. Cao, et al., “Calcium Homeostasis in the Epididymal Microenvironment: Is Extracellular Calcium a Cofactor for Matrix Gla Protein-Dependent Scavenging Regulated by Vitamins,” Front. Cell Dev. Biol., vol. 10, p. 827940, 2022, doi: 10.3389/fcell.2022.827940.
[9] J. Kazmierczak-Baranska and B. T. Karwowski, “Vitamin K Contribution to DNA Damage—Advantage or Disadvantage? A Human Health Response,” Nutrients, vol. 14, no. 20, 2022, doi: 10.3390/nu14204219.
[10] W. Fu, Q. Cui, Z. Bu, H. Shi, Q. Yang, and L. Hu, “Elevated Sperm DNA Fragmentation Is Correlated With an Increased Chromosomal Aneuploidy Rate of Miscarried Conceptus in Women of Advanced Age Undergoing Fresh Embryo Transfer Cycle,” Front. Endocrinol. (Lausanne), vol. 15, p. 1289763, 2024, doi: 10.3389/fendo.2024.1289763.
[11] T. Irez, S. Sahmay, P. Ocal, et al., “Investigation of the Association Between the Outcomes of Sperm Chromatin Condensation and Decondensation Tests, and Assisted Reproduction Techniques,” Andrologia, vol. 47, no. 4, pp. 438–447, 2015, doi: 10.1111/and.12286.
[12] M. E. Hammadeh, T. Zeginiadov, P. Rosenbaum, T. Georg, W. Schmidt, and E. Strehler, “Predictive Value of Sperm Chromatin Condensation (Aniline Blue Staining) in the Assessment of Male Fertility,” Arch. Androl., vol. 46, no. 2, pp. 99–104, 2001, doi: 10.1080/01485010117363.
[13] D. P. Evenson, “The Sperm Chromatin Structure Assay (SCSA®) and Other Sperm DNA Fragmentation Tests for Evaluation of Sperm Nuclear DNA Integrity as Related to Fertility,” Anim. Reprod. Sci., vol. 169, pp. 56–75, 2016, doi: 10.1016/j.anireprosc.2016.01.017.
[14] F. Sefid, S. Ostadhosseini, S. M. Hosseini, F. Ghazvini Zadegan, M. Pezhman, and M. H. Nasr Esfahani, “Vitamin K2 Improves Developmental Competency and Cryo-Tolerance of In Vitro Derived Ovine Blastocyst,” Cryobiology, vol. 77, pp. 34–40, 2017, doi: 10.1016/j.cryobiol.2017.06.001.
[15] K. Kaltsas, “Oxidative Stress and Male Infertility: The Protective Role of Antioxidants,” Medicina, vol. 59, no. 10, p. 1769, 2023, doi: 10.3390/medicina59101769.
[16] Y. J. R. Menezo, A. Hazout, G. Panteix, et al., “Antioxidants to Reduce Sperm DNA Fragmentation: An Unexpected Adverse Effect,” Reprod. Biomed. Online, vol. 14, no. 4, pp. 418–421, 2007, doi: 10.1016/S1472-6483(10)60887-5.
[17] K. Nowicka-Bauer and B. Nixon, “Molecular Changes Induced by Oxidative Stress That Impair Human Sperm Motility,” Antioxidants, vol. 9, no. 2, p. 134, 2020, doi: 10.3390/antiox9020134.
[18] Z. Caliskan, C. Kucukgergin, G. Aktan, A. Kadioglu, and G. Ozdemirler, “Evaluation of Sperm DNA Fragmentation in Male Infertility,” Andrologia, vol. 54, no. 11, p. e14587, 2022, doi: 10.1111/and.14587.
[19] G. Barroso, M. Morshedi, and S. Oehninger, “Analysis of DNA Fragmentation, Plasma Membrane Translocation of Phosphatidylserine and Oxidative Stress in Human Spermatozoa,” Hum. Reprod., vol. 15, no. 6, pp. 1338–1344, 2000, doi: 10.1093/humrep/15.6.1338.
[20] S. Nasrin, I. A. Ahmad, M. F. Farid, R. N. Nazari, and J. H. Hashemi, “Correlation Between Sperm Parameters and Sperm DNA Fragmentation in Fertile and Infertile Men,” Sid.ir, vol. 6, no. 1, pp. 13–18, 2008. [Online]. Available: https://sid.ir/paper/295142/en. Accessed: Oct. 19, 2024.
[21] N. G. Cassuto, A. Hazout, I. Hammoud, et al., “Correlation Between DNA Defect and Sperm-Head Morphology,” Reprod. Biomed. Online, vol. 24, no. 2, pp. 211–218, 2012, doi: 10.1016/j.rbmo.2011.10.006.
[22] A. Agarwal and T. M. Said, “Oxidative Stress, DNA Damage and Apoptosis in Male Infertility: A Clinical Approach,” BJU Int., vol. 95, no. 4, pp. 503–507, 2005, doi: 10.1111/j.1464-410X.2005.05328.x.
[23] P. Newman, F. Bonello, A. S. Wierzbicki, P. Lumb, G. F. Savidge, and M. J. Shearer, “The Uptake of Lipoprotein-Borne Phylloquinone (Vitamin K1) by Osteoblasts and Osteoblast-Like Cells: Role of Heparan Sulfate Proteoglycans and Apolipoprotein E,” J. Bone Miner. Res., vol. 17, no. 3, pp. 426–433, 2002, doi: 10.1359/jbmr.2002.17.3.426.
[24] C. M. Gundberg, J. B. Lian, and S. L. Booth, “Vitamin K-Dependent Carboxylation of Osteocalcin: Friend or Foe?” Adv. Nutr., vol. 3, no. 2, pp. 149–157, 2012, doi: 10.3945/an.112.001834.
[25] J. Li, H. Wang, and P. A. Rosenberg, “Vitamin K Prevents Oxidative Cell Death by Inhibiting Activation of 12-Lipoxygenase in Developing Oligodendrocytes,” J. Neurosci. Res., vol. 87, no. 9, pp. 1997–2005, 2009, doi: 10.1002/jnr.22029.
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