Molecular Detection of Nuc and Hib Genes of Staphylococcus Aureus Isolated from Burn Patients in Baghdad Province
DOI:
https://doi.org/10.21070/ijhsm.v2i1.158Keywords:
Staph. aureus, Burn Infections, Antibiotic Resistance, Virulence Genes, PCR DetectionAbstract
The current study was conducted to identify pathogenic Staph. Aureus that coexist with Burn Patients and characterize these bacteria based on some virulence genes using bacteriological and molecular techniques such as PCR. The study was carried out on 66 samples isolated from Burn patients in different hospitals in Iraq, Baghdad Province. These samples were cultivated on traditional and specific media agars for the identification of certain pathogenic bacteria. The results the results show that out 66 wound swabs, 55 (88.3%) of them growth Staph. aureus. The highest susceptible antibiotics against Staph. Aureus in this study were Imipenem (IPM), Cefepime (CPM), Ceftriaxone (CRO), and Cefotaxime (CTX) as (76%, 66%, 63%, and 61, respectively), while the highest resistant antibiotics were Cefixime (CFM), Vancomycin (VAN), and Amikacin (AK) as (84%, 75%, and 50%, respectively). In the current study, the molecular detection showed that 55(83.3%) of staph aureu were positive for Nuc gene. In the current study demonstrate that the S. aureus is adequate medium for growth of and production of enterotoxins.. The study findings reveal a diverse community of bacterial species present in samples isolated from patients with Burn infections with high virulence, detected by the presence of virulence genes.
Highlights:
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High Prevalence of Staph. aureus: 88.3% of wound swabs from burn patients tested positive for Staph. aureus, indicating a significant presence in burn infections.
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Antibiotic Resistance Patterns: The bacteria showed high resistance to Cefixime (84%) and Vancomycin (75%), raising concerns about treatment effectiveness.
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Virulence Gene Detection: 83.3% of Staph. aureus isolates carried the Nuc gene, highlighting their potential for high virulence and enterotoxin production.
Keywords: Staph. aureus, Burn Infections, Antibiotic Resistance, Virulence Genes, PCR Detection
References
[1] Centers for Disease Control and Prevention (CDC), “Outbreaks of Community-Associated Methicillin-Resistant Staphylococcus aureus Skin Infections—Los Angeles County, California, 2002–2003,” MMWR Morbidity and Mortality Weekly Report, vol. 52, no. 5, pp. 88, Feb. 2003.
[2] H. W. Boucher and G. R. Corey, “Epidemiology of Methicillin-Resistant Staphylococcus aureus,” Clinical Infectious Diseases, vol. 46, suppl. 5, pp. S344–S349, Jun. 2008.
[3] S. Mohapatra, A. Gupta, and K. Agrawal, “Bacteriological Profiles in Burn Patients Within First Twenty-Four Hours of Injury,” International Journal of Medical Microbiology and Tropical Diseases, vol. 2, pp. 71–73, 2016.
[4] D. P. Calfee, “The Epidemiology, Treatment, and Prevention of Transmission of Methicillin-Resistant Staphylococcus aureus,” Journal of Infusion Nursing, vol. 34, no. 6, pp. 359–364, 2011.
[5] H. H. Jubair and A. H. Khlebos, “Molecular Detection of Methicillin and Vancomycin Resistance in Staphylococcus aureus Isolated from Burn and Wound Infection Patients,” Al-Kufa University Journal for Biology, 2016.
[6] D. Pillai, C. Sheppard, and A. D. Baron, “Can People Guess What Happened to Others from Their Reactions?” PLoS ONE, vol. 7, no. 11, e49786, 2012.
[7] M. V. Gittens-St Hilaire, C. Clarke-Greenidge, and S. R. J. Springer, “Molecular Characteristics and Antimicrobial Susceptibility Patterns of MRSA in Hospitalised and Non-Hospitalised Patients in Barbados,” New Microbes and New Infections, vol. 35, pp. 100659, 2020.
[8] CLSI, “Verification of Commercial Microbial Identification and Susceptibility Test Systems, M52 Guideline,” Clinical and Laboratory Standards Institute, Wayne, PA, USA, 2016.
[9] H. M. Jasim and Z. M. Alzubaidy, “Molecular Detection of Some Virulence Genes of Methicillin-Resistant Staphylococcus aureus Clinical Isolates in Diyala Province,” Pakistan Journal of Medical & Health Sciences, vol. 16, no. 4, pp. 920–920, 2022.
[10] T. Morovat, M. Goudarzi, and A. Ghazvini, “Distribution of Different Carbapenem Resistant Clones of Acinetobacter baumannii in Tehran Hospitals,” New Microbiologica, vol. 32, no. 3, pp. 265–271, 2009
[11] Ashraf, M. A. Rasha, and A. M. El-Dougdoug, “Virulence-Associated Genes Profiling of Streptococcus iniae Isolated from Diseased Nile Tilapia (Oreochromis niloticus),” Benha Veterinary Medical Journal, vol. 42, pp. 186–190, 2022.
[12] H. A. Mohammed, A. H. Kareem, and Z. A. Salman, “Use of Multiplex PCR Technique for Distribution of Accessory Gene Regulatory Polymorphisms Among Baghdad Clinical Staphylococcus aureus Isolates and Its Correlation to Cassette Type,” World Journal of Experimental Biosciences, vol. 5, no. 1, pp. 23–29, 2017.
[13] H. Qutaiba-Dhiaa and H. R. Al-Taai, “Molecular Detection of Hospital-Acquired and Community-Acquired Staphylococcus aureus by PCR Amplification of nuc Gene, Micrococcus Species and Rothia Species,” Pakistan Journal of Medical & Health Sciences, vol. 16, no. 7, pp. 706–710, 2022.
[14] M. G. Avila-Novoa, “Detection of Enterotoxin Genes of Staphylococcus aureus Isolates from Food Contact Surfaces in the Dairy Industry of Jalisco, Mexico,” Journal of Food Protection, vol. XX, no. 2, pp. 72–78, 2018.
[15] J. Faizan, R. Kumar, and M. A. Ansari, “Molecular Typing of Staphylococcus aureus Based on Coagulase Gene,” Veterinary World, vol. 11, no. 4, pp. 423–430, 2018.
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