Critical Impact of Storage Conditions on Kidney Biomarkers
Dampak Kritis Kondisi Penyimpanan pada Biomarker Ginjal
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(1) Risma Intantri Universitas Muhammadiyah Sidoarjo
Indonesia
(2) * Andika Aliviameita
Universitas Muhammadiyah Sidoarjo
Indonesia
(3) Puspitasari Puspitasari Universitas Muhammadiyah Sidoarjo
Indonesia
(4) Chylen Setyo Rini Universitas Muhammadiyah Sidoarjo
Indonesia
(*) Corresponding Author
Abstract
This study investigated the impact of storage time (0, 4, 8, and 24 hours) at 2-8°C on urea and creatinine levels in serum samples from healthy young adults. Using a laboratory experimental design, 32 serum samples were analyzed with enzymatic and Jaffe-kinetic methods on a Microlab 300 photometer. Results from One Way ANOVA indicated significant effects on urea (p=0.007) and creatinine levels (p=0.009) across different storage times, with Post-Hoc tests revealing significant differences between the 0-hour and 24-hour storage groups for both biomarkers. These findings highlight the importance of standardized storage protocols in clinical laboratories to ensure accurate kidney function assessment and improve diagnostic reliability.
Highlights:
- Storage Conditions Influence Biomarker Levels: Temperature and time affect urea and creatinine.
- Experimental Setup: Controlled lab study on serum samples with varying storage durations.
- Clinical Importance: Standard protocols ensure accurate kidney function assessment in diagnostics..
Keywords: kidney function, serum biomarkers, storage conditions, clinical laboratories, diagnostic reliability
References
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[13] R. Meilinda, "Perbedaan Kadar Asam Urat Berdasarkan Waktu Penyimpanan Serum Selama 0 Jam, 72 Jam, Dan 96 Jam Pada Suhu Ruang," Universitas Muhammadiyah Semarang, 2017.
[14] I. Apriliani, "Perbedaan Kadar Elektrolit (Na, K, Cl) Pada Sampel Segera Dan Ditunda 150 Menit," Universitas Muhammadiyah Semarang, 2018.
[15] F. Noor, "Perbedaan Kadar Gula Darah Antara Sampel Serum, Plasma Naf Dan Plasma EDTA," Universitas Muhammadiyah Semarang, 2017.
[16] C. D. Pratiwi, H. Hariyanto, A. H. Hermawati, and I. N. Fajrin, "Pengaruh Serum yang Disimpan Selama Lima Hari Suhu 2-8°C dengan Serum yang Diperiksa Langsung pada Pemeriksaan Kolesterol Total: The Effect of Serum Stored for Five Days Temperature of 2-8°C with Serum That is Checked Directly on Total Cholesterol Testing," Borneo Journal of Medical Laboratory Technology, vol. 4, no. 2, Art. no. 2, Apr. 2022, doi: 10.33084/bjmlt.v4i2.3790.
[17] Permenkes, "Peraturan Menteri Kesehatan Republik Indonesia Nomor. 1792/Menkes/SK/XXI/2010 Tentang Pedoman Pemeriksaan Kimia Klinik Menteri Kesehatan Republik Indonesia," Jakarta, Indonesia, 2010.
[2] Q. A. N. Ramadhani, A. Garini, N. Nurhayati, and S. H. Harianja, "Perbedaan Kadar Glukosa Darah Sewaktu Menggunakan Serum Dan Plasma EDTA," JPP (Jurnal Kesehatan Poltekkes Palembang), vol. 14, no. 2, Art. no. 2, Nov. 2019, doi: 10.36086/jpp.v14i2.407.
[3] I. P. Sari, "Perbedaan Kadar Kreatinin Serum Yang Diperiksa Segera Dan Ditunda Pada Suhu Ruang," KTI Universitas Muhammadiyah Semarang, 2018.
[4] S. Wahyuni, "Gambaran Kadar Kreatinin Dan Ureum Pada Penderita Gagal Ginjal Kronik Sebelum Dan Sesudah Hemodialisa Di R.S.U Kisaran Asahan," KTI Teknologi Laboratorium Medis, Politeknik Kesehatan Kemenkes Medan, 2020, pp. 45.
[5] A. A. Alfonso, A. E. Mongan, and M. F. Memah, "Gambaran Kadar Kreatinin Serum Pada Pasien Penyakit Ginjal Kronik Stadium 5 Non Dialisis," Jurnal e-Biomedik, vol. 4, no. 1, 2016.
[6] P. N. Nuratmini, "Gambaran Kadar Ureum Dan Kreatinin Serum Pada Pasien GGK Setelah Terapi Hemodialisis Di RSD Mangusada, Kabupaten Badung," KTI, Jurusan Analis Kesehatan, Politeknik Kesehatan Kemenkes Denpasar, 2019, pp. 87.
[7] R. Melya and T. Aryani, "Evaluasi Penyimpanan Serum Berdasarkan Variasi Waktu Dan Suhu Terhadap Kadar Glukosa Dan Kreatinin," Skripsi, Program Studi Teknologi Laboratorium Medis, Fakultas Ilmu Kesehatan, Universitas Aisyiyah Yogyakarta, 2021, pp. 9.
[8] J. Maghfiroh, "Perbedaan Kadar Ureum Serum Yang Segera Diperiksa Dan Ditunda Pada Suhu Ruang," undergraduate, Universitas Muhammadiyah Semarang, 2018. Accessed: Nov. 15, 2022. [Online]. Available: http://repository.unimus.ac.id/3221/
[9] Vernekar, "Effect Of Storage And Temperature On Two Biochemical Analytes (Creatinine And Urea) In Pooled Serum Samples Stored At -20°C," Jurnal, vol. 10, no. 1, pp. 63-67, Feb. 2023, doi: 10.4103/2349-5006.198591.
[10] M. Heins, W. Heil, and W. Withold, "Storage of Serum or Whole Blood Samples? Effects of Time and Temperature on 22 Serum Analytes," Clinical Chemistry and Laboratory Medicine, vol. 33, no. 4, pp. 231–238, 1995, doi: 10.1515/cclm.1995.33.4.231.
[11] C. Selvakumar and V. Madhubala, "Effect of Sample Storage and Time Delay (Delayed Processing) on Analysis of Common Clinical Biochemical Parameters," International Journal of Clinical Biochemistry and Research, vol. 4, no. 3, pp. 295-298, 2017, doi: 10.18231/2394-6377.2017.0069.
[12] A. Marjani, "Effect of Storage Time and Temperature on Serum Analytes," American Journal of Applied Sciences, vol. 5, no. 8, pp. 1047–1051, Aug. 2008, doi: 10.3844/ajassp.2008.1047.1051.
[13] R. Meilinda, "Perbedaan Kadar Asam Urat Berdasarkan Waktu Penyimpanan Serum Selama 0 Jam, 72 Jam, Dan 96 Jam Pada Suhu Ruang," Universitas Muhammadiyah Semarang, 2017.
[14] I. Apriliani, "Perbedaan Kadar Elektrolit (Na, K, Cl) Pada Sampel Segera Dan Ditunda 150 Menit," Universitas Muhammadiyah Semarang, 2018.
[15] F. Noor, "Perbedaan Kadar Gula Darah Antara Sampel Serum, Plasma Naf Dan Plasma EDTA," Universitas Muhammadiyah Semarang, 2017.
[16] C. D. Pratiwi, H. Hariyanto, A. H. Hermawati, and I. N. Fajrin, "Pengaruh Serum yang Disimpan Selama Lima Hari Suhu 2-8°C dengan Serum yang Diperiksa Langsung pada Pemeriksaan Kolesterol Total: The Effect of Serum Stored for Five Days Temperature of 2-8°C with Serum That is Checked Directly on Total Cholesterol Testing," Borneo Journal of Medical Laboratory Technology, vol. 4, no. 2, Art. no. 2, Apr. 2022, doi: 10.33084/bjmlt.v4i2.3790.
[17] Permenkes, "Peraturan Menteri Kesehatan Republik Indonesia Nomor. 1792/Menkes/SK/XXI/2010 Tentang Pedoman Pemeriksaan Kimia Klinik Menteri Kesehatan Republik Indonesia," Jakarta, Indonesia, 2010.
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Published
2024-06-19
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