Wafaa Abdulkadhim Issa (1), Wijdan Abbas Eneama (2), Salah Naser (3)
Abstract. This study aimed to detect Human Cytomegalovirus (CMV) in patients with renal failure using RT-qPCR. A total of 100 blood and serum samples were collected from July to September 2023 from individuals aged 1 to 80 years. The RT-qPCR technique revealed 79 positive cases of CMV infection. The study population was divided into five age groups, with the 51–67 age group showing the highest infection rate (29.11%). Infections were more common in females (41 cases) than in males (38 cases). The use of real-time PCR demonstrated a rapid, sensitive, and specific diagnostic tool for CMV, especially among immunocompromised patients. These findings suggest a significant association between CMV infection and renal failure, emphasizing the need for molecular testing in clinical diagnostics. The study provides valuable epidemiological insight and supports the implementation of RT-qPCR for detecting CMV in Iraqi healthcare settings.
Highlights:
[1] M. Zuhair, G. S. A. Smit, G. Wallis, et al., "Estimation of the worldwide seroprevalence of cytomegalovirus: a systematic review and meta-analysis," Rev Med Virol, vol. 29, no. 3, pp. 1–6, 2019.
[2] J. Kim and S. Lee, “CMV and immunocompromised states,” Clin Microbiol Rev, vol. 31, no. 4, pp. e00070-18, 2018.
[3] S. E. Jackson, A. Redeker, R. Arens, et al., “CMV immune evasion and manipulation of the immune system with aging,” Geroscience, vol. 39, no. 3, pp. 273–291, 2017.
[4] R. Berry, G. M. Watson, S. Jonjic, et al., “Modulation of innate and adaptive immunity by cytomegaloviruses,” Nat Rev Immunol, vol. 20, no. 2, pp. 113–127, 2020.
[5] Z. A. Al-Fayyadh and M. N. Mezher, “Immunological and Molecular Study of Human Cytomegalovirus contribution to Anemia in patients with Chronic Kidney Disease,” Int J Pharm Res, vol. 12, pp. 2145–2151, 2020.
[6] J. Y. Feng and X. Zhang, “Post-transplant monitoring of lymphocyte counts to predict the occurrence of CMV DNAemia,” Virol J, vol. 17, no. 1, pp. 1–9, 2020.
[7]. W. Mohsin, A. H. Hassan, M. Al-Bayati, “Use of real-time PCR in CMV detection among dialysis patients,” Iraqi J Biotech, vol. 21, no. 2, pp. 55–63, 2022
[8] GoTaq qPCR Master Mix and Geneaid gSYNC DNA Extraction Kit, Manufacturer Protocols, 2023.
[9] W. Mohsin, A. H. Hassan, M. Al-Bayati, “Use of real-time PCR in CMV detection among dialysis patients,” Iraqi J Biotech, vol. 21, no. 2, pp. 55–63, 2022.
[10] J. Y. Feng and X. Zhang, “Post-transplant monitoring of lymphocyte counts to predict the occurrence of CMV DNAemia,” Virol J, vol. 17, no. 1, pp. 1–9, 2020.
[11] R. El Baba and G. Herbein, “Immune Landscape of CMV Infection in Cancer Patients: From ‘Canonical’ Diseases Toward Virus-Elicited Oncomodulation,” Front. Immunol., vol. 12, Sep. 2021, doi: 10.3389/fimmu.2021.730765.
[12] L. S. Azevedo et al., “Cytomegalovirus infection in transplant recipients,” Clinics, vol. 70, no. 7, pp. 515–523, Jul. 2015, doi: 10.6061/clinics/2015(07)09.
[13] E. Gault, Y. Michel, A. Dehée, C. Belabani, J.-C. Nicolas, and A. Garbarg-Chenon, “Quantification of Human Cytomegalovirus DNA by Real-Time PCR,” J. Clin. Microbiol., vol. 39, no. 2, pp. 772–775, Feb. 2001, doi: 10.1128/JCM.39.2.772-775.2001.
[14] J. L. Sanchez and G. A. Storch, “Multiplex, Quantitative, Real-Time PCR Assay for Cytomegalovirus and Human DNA,” J. Clin. Microbiol., vol. 40, no. 7, pp. 2381–2386, Jul. 2002, doi: 10.1128/JCM.40.7.2381-2386.2002.
[15] A. Nitsche et al., “Detection of Human Cytomegalovirus DNA by Real-Time Quantitative PCR,” J. Clin. Microbiol., vol. 38, no. 7, pp. 2734–2737, Jul. 2000, doi: 10.1128/JCM.38.7.2734-2737.2000.
[16] O. Bilenoğlu, M. Altındiş, E. Öz, Y. Y. Öz, Ö. İ. Sönmez, and C. B. Ünal, “Detailed polymorphism study on cytomegalovirus DNA polymerase gene to reveal the most suitable genomic targets for quantitative Real-time PCR,” Bosn. J. Basic Med. Sci., vol. 15, no. 3, p. 28, Jun. 2015, doi: 10.17305/bjbms.2015.494.