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Section Articles

Biosynthesized Silver Nanoparticles Demonstrate Hepatoprotective Biochemical Safety in Female Wistar Rats

Vol. 2 No. 3 (2025): Desember:
DOI : https://doi.org/10.21070/ijhsm.v2i3.350
Published : Dec 30, 2025

Suhad Khalid Sgheer (1), Ali Tariq Abdul Hassan (2)

(1) Directorate of Education of Kerbala, Ministry of Education, Kerbala, Iraq
(2) Directorate of Education of Kerbala, Ministry of Education, Kerbala, Iraq
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Abstract:

General Background: Silver nanoparticles (AgNPs) are widely applied in biomedical and technological fields, yet conventional chemical and physical synthesis methods raise safety and environmental concerns. Specific Background: Biosynthesis using probiotic bacteria such as Lactobacillus rhamnosus has emerged as a green alternative with potential biocompatibility advantages. Knowledge Gap: Limited in vivo evidence exists regarding the biochemical and histological safety of biosynthesized AgNPs, particularly their hepatic effects. Aims: This study aimed to evaluate the biochemical and histological effects of AgNPs biosynthesized by L. rhamnosus in female Wistar albino rats. Results: Biosynthesized AgNPs significantly reduced liver enzymes (ALT, AST, and ALP), significantly increased vitamin B12 levels, showed no significant change in body weight, and preserved normal liver tissue architecture. Novelty: The study demonstrates the hepatic safety profile of L. rhamnosus–mediated AgNPs in vivo compared with risks reported for conventionally synthesized AgNPs. Implications: These findings support probiotic-mediated biosynthesis as a safer and environmentally friendly approach for developing silver nanoparticles for biomedical applications.
Keywords : Biosynthesized Silver Nanoparticles, Lactobacillus rhamnosus, Green Nanoparticle Synthesis, Liver Enzyme Biomarkers, Wistar Albino Rats
Highlight :




  • Significant reductions in AST, ALT, and ALP without alterations in body mass.




  • Serum vitamin B12 concentrations increased markedly following 30-day administration.




  • Liver histology preserved normal architecture, indicating absence of hepatocellular damage.



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