Probiotic Applications of Leuconostoc Mesenteroides: Antibacterial Activity Against MDR Pathogenic Bacteria
Aplikasi Probiotik Leuconostoc Mesenteroides: Aktivitas Antibakteri Terhadap Bakteri Patogen MDR
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(1) Noor Alhuda Nadhim Hammadi Medical Biotechnology Department, College of Biotechnology, Al-Qasim Green University
Iraq
(2) * Shaimaa Obaid Hasson
Medical Biotechnology Department, College of Biotechnology, Al-Qasim Green University
Iraq
(3) Mohammed Jabber Al-Awady Medical Biotechnology Department, College of Biotechnology, Al-Qasim Green University
Iraq
(*) Corresponding Author
Abstract
Leuconostoc mesenteriodes is an important probiotic bacteria with impact characteristics and benefits for human health. Last year, probiotic bacteria were used as an alternative to traditional antibiotics in treat of different diseases especially in the broad distribution of MDR bacteria. L. mesenteriodes isolate was purchased from (Al-Amin Center for Research and Biotechnology\ Najaf, Iraq) and re-diagnosis using MRS media in anaerobic condition then diagnosis by vitek 2 system. The antibacterial activity of L. mesenteriodes was assessed using the agar well diffusion method against MDR pathogenic bacteria isolated from different clinical cases. The L. mesenteriodes crude was obtained by centrifuging the bacterial isolates. Five different concentrations of probiotic bacteria crude were tested against MDR pathogenic bacteria revealing the antibacterial activity of L. mesenteriodes crude concentrations (stock, 90%, 75%, 50% and 25% ) against MDR Staphylococcus aureus (22, 20, 20, 17, 14 mm), MDR Micrococcu. leuts (24, 23, 18, 16, 11 mm), MDR Proteus. mirabillis (22, 22, 18, 17, 14 mm), MDR Escherichia. coli (22, 20, 18, 12, 11 mm) and MDR Klebsiella pneumoniae were (20, 18, 14, 12, 11mm) an respectively. L. mesenteriodes probiotics improve antibacterial activity especially when used against multi-drug resistant bacteria
Highlights:
- mesenteroides shows antibacterial potential against MDR pathogens.
- Tested using agar well diffusion with five crude concentrations.
- Effective against MDR bacteria strains including S. aureus, E. coli, and K. pneumoniae.
Keywords: probiotic, L. mesenteriodes, MDR, Crud extract, pathogenic bacteria.
References
B. Moukafih, et al., "New Antibiotics for Multi-Drug Resistant Bacterial Strains," Cahiers Sante Medecine Therapeutique, vol. 31, no. 3, pp. 208-218, 2022.
B. Almutairy, "Extensively and Multidrug-Resistant Bacterial Strains: Case Studies of Antibiotics Resistance," Frontiers in Microbiology, vol. 15, p. 1381511, 2024.
S. Mal, et al., "Probiotics as a Therapeutic Approach for Non-Infectious Gastric Ulcer Management: A Comprehensive Review," Probiotics and Antimicrobial Proteins, pp. 1-26, 2024.
D. Hemme and C. Foucaud-Scheunemann, "Leuconostoc: Characteristics, Use in Dairy Technology and Prospects in Functional Foods," International Dairy Journal, vol. 14, no. 6, pp. 467-494, 2004.
Y. Li, et al., "Extraction and Biological Activity of Exopolysaccharide Produced by Leuconostoc Mesenteroides SN-8," International Journal of Biological Macromolecules, vol. 157, pp. 36-44, 2020.
Y. Hechard, et al., "Characterization and Purification of Mesentericin Y105, an Anti-Listeria Bacteriocin from Leuconostoc Mesenteroides," Microbiology, vol. 138, no. 12, pp. 2725-2731, 1992.
M. U. Hassan, et al., "Characterisation of Bacteriocins Produced by Lactobacillus spp. Isolated from Traditional Pakistani Yoghurt and Their Antimicrobial Activity Against Common Foodborne Pathogens," BioMed Research International, vol. 2020, no. 1, p. 8281623, 2020.
J. Bjorkroth and W. Holzapfel, "Genera Leuconostoc, Oenococcus and Weissella," The Prokaryotes, vol. 4, pp. 267-319, 2006.
F. H. Mokdad, et al., "Characterization of Bioactive Leuconostoc Mesenteroides Producing Bacteriocin Strains Isolated from Camel’s and Goat’s Algerian Raw Milks," Ponte, vol. 76, no. 3, 2020.
E. I. Garvie, "Separation of Species of the Genus Leuconostoc and Differentiation of the Leuconostocs from Other Lactic Acid Bacteria," in Methods in Microbiology, vol. 16, Academic Press, pp. 147-178, 1984.
S. Raimondi, et al., "Phylogenomic Analysis of the Genus Leuconostoc," Frontiers in Microbiology, vol. 13, p. 897656, 2022.
R. Ashraf and N. P. Shah, "Immune System Stimulation by Probiotic Microorganisms," Critical Reviews in Food Science and Nutrition, vol. 54, no. 7, pp. 938-956, 2014.
W. Rao, et al., "Antibacterial Mechanism of Metabolites of Leuconostoc Mesenteroides Against Serratia Liquefaciens," LWT, vol. 187, p. 115335, 2023.
J. Tang, L. Yi, and K. Zeng, "Isolation of Antagonistic Lactic Acid Bacteria from Traditional Fermented Foods of China and Biocontrol Mode of Leuconostoc Mesenteroides SNP037 Against Green Pepper Soft Rot Caused by Pectobacterium Carotovorum," Postharvest Biology and Technology, vol. 212, p. 112872, 2024.
E. N. D. Mahdy, S. H. M. Al-Zahrani, and Z. A. M. Al-Mahmoudi, "Partial Purification, Characterization and Antibacterial Activity of Bacteriocin from Leuconostoc Mesenteroides," International Journal of Life Science and Pharma Research, vol. 10, no. 3, pp. L6-L13, 2020.
A. Zapasnik, B. Sokolowska, and M. Bryla, "Role of Lactic Acid Bacteria in Food Preservation and Safety," Foods, vol. 11, no. 9, p. 1283, 2022.
H. Fidan, et al., "Recent Developments of Lactic Acid Bacteria and Their Metabolites on Foodborne Pathogens and Spoilage Bacteria: Facts and Gaps," Food Bioscience, vol. 47, p. 101741, 2022.
W. Rao, et al., "Antibacterial Mechanism of Metabolites of Leuconostoc Mesenteroides Against Serratia Liquefaciens," LWT, vol. 187, p. 115335, 2023.
D. J. Das, et al., "Critical Insights into Antibiotic Resistance Transferability in Probiotic Lactobacillus," Nutrition, vol. 69, p. 110567, 2020.
M. Radovanovic, et al., "Potential Influence of Antimicrobial Resistance Gene Content in Probiotic Bacteria on the Gut Resistome Ecosystems," Frontiers in Nutrition, vol. 10, p. 1054555, 2023.
L. Touza-Otero, M. Landin, and P. Diaz-Rodriguez, "Fighting Antibiotic Resistance in the Local Management of Bovine Mastitis," Biomedicine & Pharmacotherapy, vol. 170, p. 115967, 2024.
G. E. Dawwam, et al., "Activity and Downregulate Biofilm Genes of Uropathogenic E. Coli," Journal of Pure & Applied Microbiology, vol. 16, no. 3, 2022.
E. Meade, M. A. Slattery, and M. Garvey, "Bacteriocins, Potent Antimicrobial Peptides and the Fight Against Multi Drug Resistant Species: Resistance is Futile?," Antibiotics, vol. 9, no. 1, p. 32, 2020.
I. J. Adeosun, I. T. Baloyi, and S. Cosa, "Anti-Biofilm and Associated Anti-Virulence Activities of Selected Phytochemical Compounds Against Klebsiella Pneumoniae," Plants, vol. 11, no. 11, p. 1429, 2022.
M. Shaaban, et al., "Antimicrobial and Antibiofilm Activities of Probiotic Lactobacilli on Antibiotic-Resistant Proteus Mirabilis," Microorganisms, vol. 8, no. 6, p. 960, 2020.
