Antibacterial Activity of Some Lactic Acid Bacteria Isolated From Milk Products Against Streptococcus pyogenes and Staphylococcus aureus Causing Tonsillopharyngitis
Aktivitas Antibakteri Beberapa Bakteri Asam Laktat yang Diisolasi dari Produk Susu Terhadap Streptococcus pyogenes dan Staphylococcus aureus Penyebab Tonsilofaringitis
DOI:
https://doi.org/10.21070/ijhsm.v2i2.97Keywords:
Tonsillopharyngitis, Streptococcus pyogenes, Staphylococcus aureus, bacilli and lactobacilli, auto-aggregation, co-aggregation.Abstract
Background: Tonsillitis and pharyngitis are the most common oral infections accompanied by risky complications, including infection recurrence and antibiotic resistance. Streptococcus pyogenes and Staphylococcus aureus are the predominant cause of such infection. Objective: This study comes to investigate the nature of bacterial interactions between probiotic lactobacilli and bacilli, isolated from milk products, with the most common pathogenic bacteria causing tonsillitis and pharyngitis in human. Methods: A 20 samples of local milk products were collected. in addition to 25 tonsil swabs were collected from patients with tonsillopharyngitis. VITEK system2 was used to confirm bacterial identification. The antibiotic susceptibility of bacterial isolates was evaluated to five tested antibiotics. Co-aggregation capability of tested bacterial species was evaluated to investigate the nature of bacterial interactions towards each other. Results: Of the 20 milk samples, the VITEK2 system confirmed that 3 (60%) of bacterial growth were identified as Bacillus subtillus while 8 (72.7%) were Lactobacillus plantarum. On the other hand, 11 (73.3%) of bacterial growth were Streptococcus pyogenes and 6 (100%) were Staphylococcus aureus isolated from tonsillopharngitis patients. The isolated S. pyogenes and S. aureus were highly resistant to the tested antibiotics, but not to vancomycin. Both bacilli and lactobacilli species showed high co-aggregation scores with the isolated pathogenic bacteria. Conclusions: The antibiotic resistance of pathogenic bacteria requires urgent, safe, and effective alternative antimicrobial agents. Beneficial bacteria capable to compete with the growth of pathogens and inhibit their growth, eventually, preventing such infections.
Highlights:
- Antibiotic-resistant S. pyogenes and S. aureus cause recurrent tonsillopharyngitis.
- Milk-derived L. plantarum and B. subtilis co-aggregate with pathogens, inhibiting colonization.
- Probiotics offer a natural alternative for preventing antibiotic-resistant infections.
Keywords: Tonsillopharyngitis, Streptococcus pyogenes, Staphylococcus aureus, bacilli and lactobacilli, auto-aggregation and co-aggregation.
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[21] S. Karami, M. Roayaei, H. Hamzavi, M. Bahmani, H. Hassanzad-Azar, L. M., et al., "Isolation and Identification of Probiotic Lactobacillus from Local Dairy and Evaluating Their Antagonistic Effect on Pathogens," International Journal of Pharmaceutical Investigation, vol. 7, no. 3, pp. 137-141, 2017.
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[28] D. Ramesh, S. Souissi, and T. S. Ahamed, "Effects of the Potential Probiotics Bacillus aerophilus KADR3 in Inducing Immunity and Disease Resistance in Labeo rohita," Fish & Shellfish Immunology, vol. 70, pp. 408-415, 2017.
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[30] D. Das and A. Goyal, "Antioxidant Activity and γ-Aminobutyric Acid (GABA) Producing Ability of Probiotic Lactobacillus plantarum DM5 Isolated from Marcha of Sikkim," LWT - Food Science and Technology, vol. 61, no. 1, pp. 263-268, 2015.
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[32] D. Kebede, A. Admas, and D. Mekonnen, "Prevalence and Antibiotic Susceptibility Profiles of Streptococcus pyogenes Among Pediatric Patients with Acute Pharyngitis at Felege Hiwot Comprehensive Specialized Hospital, Northwest Ethiopia," BMC Microbiology, vol. 21, no. 1, p. 135, 2021.
[33] H. M. Fahad, "Types of Aerobic Bacteria Isolated from Iraqi Patients with Acute Tonsillitis and Their Susceptibility to Different Antibiotics," Journal of Pure and Applied Microbiology, vol. 12, no. 4, pp. 1855-1859, 2018.
[34] C. Timon, V. McAllister, M. Walsh, and M. Cafferkey, "Changes in Tonsillar Bacteriology of Recurrent Acute Tonsillitis: 1980 vs. 1989," Respiratory Medicine, vol. 84, no. 5, pp. 395-400, 1990.
[35] H. H. Darod, A. Melese, M. Kibret, and W. Mulu, "Bacterial Tonsillitis and Antimicrobial Resistance Profiles Among Children within Five Years of Age at Hargeisa Group of Hospital, Somaliland: A Cross-Sectional Study," 2021.
[36] Z. Heussien, N. Yousef, H. Temark, and A. Ebnawaled, "Isolation, Identification and Antibiogram Study of Pathogenic Bacteria Mediated Children Chronic Tonsillitis at Qena University Hospital, Egypt," SVU International Journal of Medical Sciences, vol. 3, no. 2, pp. 1-8, 2020.
[37] U. Babaiwa, N. Onyeagwara, and J. Akerele, "Bacterial Tonsillar Microbiota and Antibiogram in Recurrent Tonsillitis," Biomedical Research, vol. 24, no. 3, pp. 298-302, 2013.
[38] S. Bakir and F. Ali, "Evaluation of Multi-Drug Resistance and β-Lactamase Production in Throat Infected by Gram-Positive Bacteria," European Journal of Pharmaceutical Sciences, vol. 3, no. 2, pp. 68-76, 2016.
[39] N. M. Wijesundara and H. V. Rupasinghe, "Herbal Tea for the Management of Pharyngitis: Inhibition of Streptococcus pyogenes Growth and Biofilm Formation by Herbal Infusions," Biomedicines, vol. 7, no. 3, p. 63, 2019.
[40] A. Agrawal, D. Kumar, A. Goyal, R. Gupta, and S. Bhooshan, "Bacteriological Evaluation and Their Antibiotic Sensitivity Pattern in Tonsillitis," IOSR Journal of Dental and Medical Sciences, vol. 13, no. 3, pp. 51-55, 2014.
[41] A. Reta, A. B. Kifilie, and A. Mengist, "Bacterial Infections and Their Antibiotic Resistance Pattern in Ethiopia: A Systematic Review," Preventive Medicine, vol. 2019, p. 4380309, 2019.
[42] M. E. Pichichero, "Explanations and Therapies for Penicillin Failure in Streptococcal Pharyngitis," Clinical Pediatrics, vol. 31, pp. 642-649, 1992.
[43] G. S. Ståhlgren et al., "Penicillin V Four Times Daily for Five Days Versus Three Times Daily for 10 Days in Patients with Pharyngotonsillitis Caused by Group A Streptococci: Randomised Controlled, Open Label, Non-Inferiority Study," BMJ, vol. 367, p. l5337, 2019.
[44] K. Hedin, S. Thorning, and M. L. van Driel, "Different Antibiotic Treatments for Group A Streptococcal Pharyngitis," Cochrane Database of Systematic Reviews, vol. 3, no. 3, p. CD004406, 2021.
[45] R. S. Gruchalla and M. Pirmohamed, "Clinical Practice: Antibiotic Allergy," New England Journal of Medicine, vol. 354, no. 6, pp. 601-609, 2006.
[46] A. Berwal, K. Chawla, S. Shetty, and A. Gupta, "Trend of Antibiotic Susceptibility of Streptococcus Pyogenes Isolated from Respiratory Tract Infections in a Tertiary Care Hospital in South Karnataka," Iranian Journal of Medical Microbiology, vol. 11, no. 1, pp. 13-18, 2019.
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Published
2025-02-15
How to Cite
Naser, R. H. ., Algburi, A. R. ., & Popov, I. . (2025). Antibacterial Activity of Some Lactic Acid Bacteria Isolated From Milk Products Against Streptococcus pyogenes and Staphylococcus aureus Causing Tonsillopharyngitis: Aktivitas Antibakteri Beberapa Bakteri Asam Laktat yang Diisolasi dari Produk Susu Terhadap Streptococcus pyogenes dan Staphylococcus aureus Penyebab Tonsilofaringitis. Indonesian Journal on Health Science and Medicine, 2(2), 10.21070/ijhsm.v2i2.97. https://doi.org/10.21070/ijhsm.v2i2.97
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