Nano-Extract of Epimedium alpinum Preserves Testicular Histology During Levofloxacin Exposure

• prof.Dr.Jabbar Abadi Mohammed

  Kufa University /Faculty of scinces

• prof.Dr.Sukayna Jabbar Mushattat

  Kufa University /Faculty of scinces

• Eelaf kareem abd ali

  Kufa University /Faculty of Education for Women

• Saja Hassan Abdul- Kadhim

  Kufa University / Faculty of Nursing

General Background: Antibiotics such as levofloxacin are widely prescribed but have been associated with reproductive tissue alterations following prolonged administration. Specific Background: Plant-derived nanomaterials, particularly nano-extracts from medicinal plants, have gained attention due to their bioactive compounds with antioxidant and anti-inflammatory properties. Knowledge Gap: Limited experimental evidence exists regarding the histological response of testicular tissue to concurrent administration of levofloxacin and Epimedium alpinum nano-extract. Aim: This study examined the histological structure of testes in mice treated with levofloxacin and varying concentrations (5 and 10 mg/kg) of Epimedium alpinum nano-extract. Results: Levofloxacin administration for 30 days resulted in seminiferous tubule degeneration, reduced sperm count, and structural disruption, whereas groups receiving the nano-extract alone maintained normal architecture. Co-administration groups demonstrated preservation of seminiferous tubule integrity and improved spermatogenic organization. Novelty: The study provides experimental histological evidence supporting the tissue-preserving role of Epimedium alpinum nano-extract against antibiotic-associated alterations. Implications: These findings highlight the potential application of plant-based nanophytopharmaceuticals as supportive agents in treatments involving antibiotics with reproductive risks.
Hightlights:

• Levofloxacin administration induced seminiferous tubule degeneration and reduced sperm count.

• Epimedium alpinum nano-extract maintained normal testicular architecture.

• Combined treatment preserved spermatogenic organization during antibiotic exposure.

Keywords: Epimedium Alpinum, Nano-Extract, Levofloxacin, Testicular Histology, Spermatogenesis

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