Relationship Between Pitx2 Snps with Weights and Body Measurements in Goat

• Shaimaa G. A. Al-Rubaye

  Department of Agricultural Biotechnology, Kut-Technical Institute, Middle Technical University

• Zainab Hussein Kareem Al-Zubaidi

  Department of Agricultural Biotechnology, Kut-Technical Institute, Middle Technical University

• Huda Muhanna Redha Redha

  Al-Suwaira Technical Institute, Middle Technical University

• Ali Ahmed Kalaf

  Department of Agricultural Biotechnology, Kut-Technical Institute, Middle Technical University

This study was conducted at the Ruminant Research Station, affiliated with the Ministry of Agriculture, from December 1, 2021, to March 5, 2022. The objective was to investigate the relationship between single nucleotide polymorphisms (SNPs) in the Paired-like homeodomain transcription factor A (PITX1) gene and growth traits, including body measurements, in goats. A total of 52 female goats were used, including 16 native and 36 Shami goats. DNA sequencing of a 966 bp fragment from the promoter region of the PITX1 gene revealed four mutations. The SNPs G1198A and G1089A exhibited two genotypes: wild-type GG and heterozygous GA, with the mutant homozygous genotype AA not detected. For the C1194T SNP (referred to in the original text as ClYIT), three genotypes were identified: CC (wild), CT (heterozygous), and TT (mutant). Another SNP, G1003A, presented all three genotypes: GG, GA, and AA. The results showed that birth weight and body measurements, such as heart girth and body length, were significantly affected by the C1194T mutation and varied among genotypes. Most other growth traits, however, were not significantly influenced by breed differences. In detail, the highest dam birth weight was observed in the CC genotype (3.23 kg), while the CT genotype was associated with lower neonate birth weight (3.19 kg). The TT genotype (mutant) recorded the highest values for heart girth (83.4 cm) and body length (79.45 cm) in neonates. Furthermore, the G1003A mutation significantly increased dam birth weight, reaching 3.55 kg in individuals with the mutant AA genotype, although it had no significant effect on other growth traits or body dimensions.

Highlights:

1. PITX1 SNPs identified: Four mutations were found in the promoter region, notably C1194T and G1003A.

2. Growth traits affected: C1194T and G1003A SNPs significantly influenced birth weight and body measurements.

3. Genotype impact varies: TT and AA genotypes showed superior values in specific growth traits.

Keywords: Pitx2, Goat, Snps, Growth, Traits

[1] M. Rotoriga, J. Li, B. Zhang, and Y. Wang, "Advances in Molecular Genetic Mapping and Marker-Assisted Selection in Animal Breeding," Animal Genetics, vol. 44, no. 2, pp. 150–158, 2013.

[2] S. A. Alakilli, K. A. El-Razik, and S. Z. El-Zarkouny, "Genetic Evaluation of Some Productive and Reproductive Traits in Friesian Cattle Under Upper Egypt Conditions," Egyptian Journal of Animal Production, vol. 49, no. 2, pp. 123–130, 2012.

[3] F. Syeda, S. Mahmood, and M. A. Khan, "Expression Analysis of PITX Genes and Their Roles in Development and Organogenesis," Gene Reports, vol. 6, pp. 36–42, 2017.

[4] A. Torlopp, T. A. Heanue, and V. Pachnis, "Pitx2 and Its Role in Early Embryonic Development and Organ Laterality," Developmental Dynamics, vol. 243, no. 4, pp. 500–511, 2014.

[5] N. Thonore, M. Duval, and M. Garcia, "Role of PITX-MYOD Interaction in Myogenesis and Muscle Fiber Specification," Developmental Biology, vol. 345, no. 1, pp. 95–105, 2010.

[6] M. Seifi, J. Fayazi, and S. Nazari, "Association of PITX1 Gene Polymorphisms With Carcass Traits in Iranian Sheep Breeds," Journal of Livestock Science and Technologies, vol. 6, no. 2, pp. 45–51, 2018.

[7] Y. Jiang, M. Xie, W. Chen, R. Talbot, and Y. Li, "The Sheep Genome Illuminates Biology of the Rumen and Lipid Metabolism," Science, vol. 361, no. 6406, p. eaat5061, 2018.

[8] X. Ding, J. Yang, Y. Wang, and Y. Zhang, "Association Analysis of PITX1 Gene With Carcass and Meat Quality Traits in Sheep," Small Ruminant Research, vol. 149, pp. 57–62, 2017.

[9] X. Zhao, Q. Meng, Q. Wang, and X. Li, "Genetic Polymorphism of the PITX1 Gene and Its Association With Growth Traits in Cattle," Molecular Biology Reports, vol. 37, no. 7, pp. 4037–4043, 2010.

[10] S. M. Dans, M. H. Khalil, and S. M. Ahmed, "Association of PITX1 Gene Polymorphisms With Growth Traits in Goats," Asian-Australasian Journal of Animal Sciences, vol. 23, no. 9, pp. 1173–1178, 2010.

[11] X. Y. Lan, C. Y. Pan, H. Chen, and C. Z. Lei, "Association of Polymorphism in the PITX1 Gene With Growth Traits in Goats," Journal of Genetics and Genomics, vol. 37, no. 12, pp. 765–770, 2010.

[12] A. Anaam, N. A. Al-Anbari, and M. T. Jassim, "Evaluation of Productive and Reproductive Performance of Some Exotic and Local Goat Breeds Under Iraqi Conditions," Iraqi Journal of Agricultural Sciences, vol. 46, no. 3, pp. 345–352, 2015.

[13] T. J. Simeon and J. O. Adewale, "Body Measurements and Growth Performance in Different Goat Breeds Under Tropical Conditions," Small Ruminant Research, vol. 146, pp. 12–18, 2017.

[14] SAS Institute Inc., Statistical Analysis System: User’s Guide, Version 9.1, 9th ed. Cary, NC: SAS Institute Inc., 2012.

[15] S. Jimmy, D. Mutettka, R. D. Kugonza, and W. Mpai, "Variability in Body Morphometric Measurements and Their Application in Predicting Live Body Weight of Mubende and Small East African Breeds in Uganda," Middle East Journal of Scientific Research, vol. 561, pp. 98–105, 2000.

[16] A. H. Salim, "Some Genetic and Non-Genetic Factors Affecting Body Dimensions at Birth and Weaning Ages for Local and Shami Goats," Journal of Karbala Agricultural Sciences, vol. 3, no. 2, pp. 12–21, 2016.

[17] A. A. Mnati, S. Said, and S. A. Taha, "Some Factors Affecting Body Weight at Birth, Weaning, and Nine Months in Cyprus Local Goats and Their Crosses," Journal of Karbala Agricultural Sciences, vol. 2, no. 4, pp. 59–75, 2015.

[18] K. Y. Merkhan, "Milk Production and Body Weight at Weaning and Their Relationships With Body and Udder Measurements in Meriz Goats," Iraqi Journal of Agricultural Sciences, vol. 50, no. 1, pp. 480–486, 2019.

[19] X. Zhao, "Impact of Genetic Factors on Body Dimensions and Weight Traits in Livestock," Journal of Animal Science and Technology, vol. 61, no. 4, pp. 34–40, 2013.

[20] H. Khan, F. Muhammad, R. Ahmed, G. Nawaz, Rahimullah, and M. Zubair, "Relationship of Body Weight With Linear Body Measurements in Goats," Journal of Agriculture and Biological Science, vol. 1, no. 3, pp. 51–54, 2006.

[21] D. D. Radhi, R. H. A. Al-Aidi, and A. A. Khalaf, "An Impact of Intravenous Injection of L-Carnitine on the Sexual Characteristics of Aged Sheep," Journal of Animal Health and Production, vol. 12, suppl. 1, pp. 258–262, 2024.