Cisplatin-induced Genotoxicity and Protective Effect of Aqueous Ginkgo Biloba Extract in an Animal Model
Genotoksisitas yang Diinduksi Cisplatin dan Efek Perlindungan Ekstrak Ginkgo Biloba Berair dalam Model Hewan
DOI:
https://doi.org/10.21070/ijhsm.v2i1.145Keywords:
Crocus sativus, Cisplatin, Sperm head abnormalities, Micronucleus TestsAbstract
It has recently been proven that many natural compounds made from crude plant extracts offer protection from the passive effects of various contaminants. One common spice and therapeutic herb is Gingko Biloba Extract. To study the effect of aqueous Gingko Biloba Extract in inhibiting the genotoxicity of cisplatin, we gave the first group cisplatin 10mg/kg only. During the work, the second group was treated with an aqueous extract of Gingko Biloba Extract 50mg/kg then 10mg/kg cisplatin. The third group was treated with an aqueous extract of Gingko Biloba Extract 100mg/kg then cisplatin 10mg/kg. On the other hand, the last group was treated with the same aqueous extract 150mg/kg, then 10mg/kg cisplatin. To perform genetic tests, we used micronuclei and sperm abnormality tests. After the treatment with cisplatin, the micronuclei and sperm abnormality were induced; however, the treatment with aqueous extract of Gingko Biloba Extract, the micronuclei and sperm abnormality were significantly reduced in male mice. These results demonstrated that cisplatin treatment alone is not effective as cisplatin treatment in combination with aqueous extract of Gingko Biloba Extract in reducing the amount of sperm head abnormalities and micronucleus.
Highlights:
- Groups: Cisplatin alone; Ginkgo 50, 100, 150 mg/kg + cisplatin.
- Tests: Micronucleus and sperm abnormality tests performed on male mice.
- Result: Ginkgo reduced cisplatin-induced micronuclei, sperm abnormalities significantly.
Keywords: Crocus sativus, Cisplatin, Sperm head abnormalities, Micronucleus Tests
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